The evaluation of the Theraputic Effect of Aqueous Extract of Aloe Vera on Entamoeba histolytica in Vivo
DOI:
https://doi.org/10.31185/wjps.524Keywords:
E.histolytica , Ale vera, metronidazoleAbstract
Ameobiasis caused by Entameoba spp. It is a zoonotic disease that is the most prevalent. Thus, the study was conducted to evaluate the anti-ameobiasis efficacy of aqueous Aloe vera extracts in comparison with metronidazole in laboratory mice. Stool samples were collected from patients attending in Al-karamah teching Hospital suffering from diarrhea from November 1, 2022 to February 29, 2023, and 15samples were examined for both sexes microscopic by using the lugols iodine Stain to detect infected the parasite's cysts , Isolation and purification by flotation with normal solution and preserved in potassium dichromate for the purpose of using it in causing infection in laboratory mice. The experimental study was conducted on groups of 35 mice by oral administration of parasite cysts within the range of 95 cyst / ml except for the negative control group that was drenched with physiological saline. The feces of mice inoculated with cysts of parasites were microscopically examined using the modified lugols iodine Stain,
After the mice were divided into seven groups with the uninfected and untreated group kept as a healthy negative control. The first group which included 21 mice was treated after it was divided
into three subgroups A, B, C for each secondary group 7 mice they were treated with aqueous extract of A.vera at different concentrations 50, 100, 150 mg/ml on the respectively, while the second group which included 21 mice on three groups A, B, and C was treated with aqueous extract of A.vera at the previous concentrations for three consecutive days for each concentration. The third group was treated with metronidazole at a concentration of mg/ ml, and the positive control group remained infected with the parasite and was not treated. After treatment a microscopic examination was performed by evaluating the excretion average of parasite cysts using a hematocytometer slide.
There was a decrease in the shedding of fecal cysts in the groups treated with aqueous extract at different concentrations, reaching a complete stop when treating with the highest concentrations at the end of the treatment period. Where, the average of shedding of fecal parasite cysts of groups of mice at concentrations 50, 100, 150 mg/ml reached 18.20, 0.200, 0 cyst/ml respectively. while the avrage of shedding of cysts in the group treated with metronidazole reached 60.20 cyst/ml in comparison with the infected positive control group in which the number of cysts continued to increase to 14.863 cyst/ml at the end of the treatment period. The results of the therapeutic efficacy of A.vera extract showed percentages of 82%, 90%, 100% for the aqueous extract at concentrations 50, 100, 150 mg/ml, respectively, while the therapeutic efficacy of metranidazol reached 84% at the end of the treatment period
References
S. Roure et al., “Approach to amoebic colitis: Epidemiological, clinical and diagnostic considerations in a non-endemic context (Barcelona, 2007-2017),” PLoS One, vol. 14, no. 2, p. e0212791, 2019.
I. K. M. Ali et al., “Proteomic Analysis of the Cyst Stage of Entamoeba histolytica,” vol. 6, no. 5, 2012, doi: 10.1371/journal.pntd.0001643.
Magda A. Ali, “A Comparison of use of Microscopic Examination and PCR for Detection of Entamoeba Histolytica at Wasit Province,” Indian J. Forensic Med. Toxicol., vol. 15, no. 4, pp. 548–551, 2021, doi: 10.37506/ijfmt.v15i4.16759.
H. V. Smith and R. A. B. Nichols, “15 Preparation of Parasitic Specimens for Direct Molecular Applications,” Handb. Nucleic Acid Purif., p. 317, 2009.
M. A. H. Mehdi, G. M. N. Omar, M. Farooqui, and V. Pradhan, “Therapeutic effect of Tamarindus Indica extracts on the pathogenesis of Entamoeba histolytica in-vivo,” Int. J. Pharm. Sci. Res., vol. 10, no. 7, pp. 3250–3256, 2019.
A. K. Kadhim and M. T. S. Al-Zubaidi, “Isolation of Cryptosporidium oocysts from slaughtered broiler chicken and Experimental infection in chicks,” Basrah J. Vet. Res., vol. 17, no. 3, pp. 703–720, 2018.
M. Bellahcene et al., “Male mice that lack the G-protein-coupled receptor GPR41 have low energy expenditure and increased body fat content,” Br. J. Nutr., vol. 109, no. 10, pp. 1755–1764, 2013.
J. Bala, “Contribution of SPSS in Social Sciences Research.,” Int. J. Adv. Res. Comput. Sci., vol. 7, no. 6, 2016.
A. M. A.-A. Mona, “Molecular epidemiology of Entamoeba Histolytica, Entamoeba Dispar and Entamoeba Moshkovskii infections in rural yemen/Mona Abdullah Mohammed Al-Areeqi.” University of Malaya, 2018.
A. S. Kadhim, S. B. Ahmad, M. Y. Owoyemi, and M. Ahmad, “Islamic ethics: The attributes of Al-Ihsan in the Quran and its effects on muslim morality,” Int. J. Bus. Soc. Sci., vol. 8, no. 11, pp. 1933–2219, 2017.
C. Troeger et al., “Estimates of the global, regional, and national morbidity, mortality, and aetiologies of lower respiratory infections in 195 countries, 1990–2016: a systematic analysis for the Global Burden of Disease Study 2016,” Lancet Infect. Dis., vol. 18, no. 11, pp. 1191–1210, 2018.
A. K. Al-Sultany and K. A. Al-Morshidy, “An epidemiological study of intestinal parasites in children attending the pediatric teaching hospital in the holy city of Karbala, Iraq,” Med. J. Babylon, vol. 20, no. 1, pp. 95–100, 2023.
A. B. M. Al-Waaly and H. W. K. Shubber, “Epidemiological study of cutaneous leishmaniasis in Al–diwaniyah province, Iraq,” EurAsian J. Biosci., vol. 14, no. 1, pp. 269–273, 2020.
M. J. Shakir and A. A. Hussein, “Journal homepage: http://www. journalijar. com INTERNATIONAL JOURNAL OF ADVANCED RESEARCH RESEARCH ARTICLE,” Int. J., vol. 2, no. 8, pp. 332–337, 2014.
Z. K. A. Al-Salehy and Z. A. A. Mohammad, “RELATIONSHIP BETWEEN FECAL CALPROTECTIN AND OTHER IMMUNOLOGICAL PARAMETERS IN DIARRHEAL CHILDREN INFECTED WITH ENTAMOEBA HISTOLYTICA AND PATHOGENIC BACTERIA IN THI-QAR PROVINCE, IRAQ,” Plant Arch., vol. 20, no. 1, pp. 1089–1095, 2020.
M. I. J. Ibrahim, S. M. Sapuan, E. S. Zainudin, and M. Y. M. Zuhri, “Extraction, chemical composition, and characterization of potential lignocellulosic biomasses and polymers from corn plant parts,” BioResources, vol. 14, no. 3, pp. 6485–6500, 2019.
Z. S. Hadi, “A Study of prevalence of intestinal parasitic infection in Shatrah district/Thi-Qar governorate,” J. Educ. pure Sci. Univ. Thi-Qar, vol. 1, no. 5, pp. 28–39, 2011.
A. Obadiah, G. A. Swaroopa, S. V. Kumar, K. R. Jeganathan, and A. Ramasubbu, “Biodiesel production from palm oil using calcined waste animal bone as catalyst,” Bioresour. Technol., vol. 116, pp. 512–516, 2012.
J. S. Akingbade, “Students’ perception of the availability and utilization of information and communication technology (ICT) in the teaching and learning of science subjects in secondary schools in Ekiti State, Nigeria,” Int. J. Educ. Lit. Stud., vol. 1, no. 1, pp. 94–99, 2013.
T. T. Mbagwu, J. K. Abioye, and B. Seye, “Prevalence of Entamoeba histolytica in Bingham University and environs,” EC Microbiol., vol. 15, no. 4, pp. 242–250, 2019.
R. Ngui et al., “Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia,” Parasit. Vectors, vol. 5, pp. 1–7, 2012.
D. R. Becker, R. E. Drake, and G. R. Bond, “Benchmark outcomes in supported employment,” Am. J. Psychiatr. Rehabil., vol. 14, no. 3, pp. 230–236, 2011.
D. Usuda et al., “Amebic liver abscess by Entamoeba histolytica,” World J. Clin. cases, vol. 10, no. 36, p. 13157, 2022.
R. Bhat and L. Steinman, “Innate and adaptive autoimmunity directed to the central nervous system,” Neuron, vol. 64, no. 1, pp. 123–132, 2009.
S. Fujishima et al., “Involvement of IL-17F via the induction of IL-6 in psoriasis,” Arch. Dermatol. Res., vol. 302, pp. 499–505, 2010.
R. Echeverría et al., “Levels and determinants of adipose tissue cadmium concentrations in an adult cohort from Southern Spain,” Sci. Total Environ., vol. 670, pp. 1028–1036, 2019.
M. Moazeni et al., “Estimation of health risks caused by exposure to enteroviruses from agricultural application of wastewater effluents,” Water Res., vol. 125, pp. 104–113, 2017.
K. Bakr, T. Abdel-Fattah, M. Shaaban, A. Diab, H. Helmi, and H. M. El-Sayed, “Stratigraphic and Petrophysical assessment of ASL sandstone reservoir in northern and central October Field, Gulf of Suez,” NRIAG J. Astron. Geophys., vol. 13, no. 1, pp. 26–45, 2024.
X. Liu, P. He, W. Chen, and J. Gao, “Improving multi-task deep neural networks via knowledge distillation for natural language understanding,” arXiv Prepr. arXiv1904.09482, 2019.
A. J. Pegu and M. A. Sharma, “Review on Aloe vera,” Int J Trend Sci Res Dev, vol. 3, no. 4, pp. 35–40, 2019.
G. M. Kadry, M. A. M. Ismail, N. M. El-sayed, H. S. El-kholy, and D. M. H. El-akkad, “In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp . cysts,” J. Parasit. Dis., vol. 45, no. 1, pp. 159–168, 2021, doi: 10.1007/s12639-020-01292-8.
R. Kadry, A. S. Newsome, and P. R. Somanath, “Pharmacological inhibition of MMP3 as a potential therapeutic option for COVID-19 associated acute respiratory distress syndrome,” Infect. Disord. Drug Targets, vol. 21, no. 6, p. e170721187996, 2021.
S. Dahhaam and S. A. Mohammed, “EFFECT OF AQUEOUS AND ALCOHOLIC EXTRACTS OF ALOE VERA ON RATS EXPERIMENTALLY INFECTED WITH ENTAMOEBA,” vol. 3, no. 07, pp. 33–44, 2022.
A. O. Adeeyo, J. N. Edokpayi, M. A. Alabi, T. A. M. Msagati, and J. O. Odiyo, “Plant active products and emerging interventions in water potabilisation: disinfection and multi-drug resistant pathogen treatment,” Clin. Phytoscience, vol. 7, pp. 1–16, 2021.
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