A Study of the Characteristics and Properties of P. aeruginosa Isolated from Different Sources and their Resistance to Antibiotics

Abstract

Pseudomonas aeruginosa, a common opportunistic bacterium, is a major source of human surface infections. Infections with these bacteria are difficult to cure and cause significant economic implications. Objectives The current study looked at the antibiotic resistance patterns of P. aeruginosa isolated from burns, wound swabs, and urinary tract infections that were referred to Iraq Hospitals' emergency health care units. From October 2022 to May 2023, 180 swabs were collected from burn and wound patients (110 burns, 67 wounds, and 3 urinary tract infections) of both sexes, (80) males and (100) females, aged 1-72 years, at Baghdad government hospitals: Ghazi Al-Hariri Hospital, Central Laboratories, Specialized Burns Hospital, and Yarmouk Hospital. Results:(180) diagnostic swabs from patients with burns and wound infections of both sexes, (80) males and (100) females, aged 1–72 years. The current study found 25(31.25) more P. aeruginosa positive cases in males than females, with 29(29) out of 54 positive cases. The study discovered that the age group 21-30 years had the highest rate of bacterial infection, followed by the age groups 11-20 and 1-10, with 11 (20.37%), 10 (18.51%), and 10 (18.51%), respectively. According to the current study's findings, the antibiotics Amikacin, Gentamicin, Ceftazidime, Cefepime, Netilmicin, and Tobramycin showed the highest resistance rates (81.4%, 81.4%, 79.6%, 72.2%, 74%, and 79.6%, respectively).The test findings showed that 52 isolates (96%) produced pyrocyanin and hemolysins. Bacterial colonies were white to gray with a sticky feel. All isolates exhibited β-hemolysis.Alkaline protease production All isolates exhibited a translucent halo surrounding the bacterial colonies.Lipase production  All isolates exhibited a hazy halo surrounding the development zone of P.aeruginosa colonies. The current study discovered that the antibiotics Amikacin, Gentamicin, Ceftazidime, Cefepime, Netilmicin, and Tobramycin were the most resistant in cases of burn and wound patients, as well as urinary tract infections in Iraq.