In vitro Investigation of the Effects of Various Media and Eucalyotus Camaldulensis Essential oil on Dermatophyte spp. Growth

Authors

  • Haider AbdHummadi Minstry of Health, IRAQ

DOI:

https://doi.org/10.31185/wjps.466

Keywords:

Culture media, Dermatophyte, Eucalyptus, Essential oil

Abstract

A Significant essential oil is Eucalyptus oil (C10H18O). Fresh and dried leaves, as well as branch tips, are used to extract the oil. Bioactive properties, and a length history of usage against many disease make eucalyptus oil a valuable medicinal tool. The results showed that the essential oil considerably affected Trichophyton rubrum's growth in liquid medium (SDB), and that impact developed as concentration increased. In comparison to the control, which weighed 4.87 gm, the weight of the mycelium mat was 1.17, 2.83, 3.57 gm at concentrations of 100, 50, and 25%, respectively. The lowest weight was found at concentration 25%, which was 3.57 gm. The concentration of 100 percent yielded the most extreme level of inhibition (76.13%), while the concentration of 25% yielded the lowest level of inhibition (26.54%). The medium SDA showed the most noteworthy growth, with radial growth of 76.67 mm following 7 days, trailed by the medium PDA at 70.33 mm. The case of the medium YEA 20.33 mm showed the most minimal growth rate. A statistical analysis uncovered that there is no significant difference (P < 0.05) between the medium MEA and YEA, but there is one among SDA and every single tried medium. subsequently the accompanying tests can be performed utilizing the medium SDA.

References

C. Eduardo Dei, "Parasitic adaptation of pathogenic fungi to mammalian hosts," CRC critical reviews in microbiolog., 13(2), 173-218, 1986A.

L. Ji. Kai, "N-containing compounds of macromycetes," Chemical reviews., 105(7), 2723-2744, 2005.

Dahdah, M. J., and R. K. Scher, "Dermatophytes Current Fungal Infection Reports," 2(2), 81-86, 2008.

M. Zarrin1, M. Poosashkan1, A. Z. Mahmoudabadi, and, M. Ali Mapar, "Prevalence of superficial fungal infection in primary school children in Ahvaz, Iran," Macedonian Journal of Medical Sciences.,‏ 4(1),89-92, 2011.

O. Dirk, M. Welle, and U. Kelter, Merging UML documents. Internal Report, University of Siegen, 2004.‏

Patil, Vishin A., and S. A. Nitave. "A review on Eucalyptus globulus: A divine medicinal herb," World journal of pharmacy and pharmaceutical sciences.3(6), 559-67, 2014.

Dutta, Smita D. "Determination of Antifungal Effect of Natural Oil and Synthetic Gutta Percha Solvents Against Candida Albicans: A Disc Diffusion Assay," Journal of Pharmacy and Bioallied Sciences., 15. Suppl 1, S235-S238,2023.

Sharma, Arun Dev, "Eucalyptol (1, 8 cineole) from eucalyptus essential oil a potential inhibitor of COVID 19 corona virus infection by molecular docking studies," (2020).‏

M. Mondal, C. Quispe, C. Sarkar, T. Chandra Bepari, Md. Jahir Alam, S. Saha, Pranta Ray, M. Rahim, M. T. Islam, M. S. Torequl, W. N. Setzer, and S. K. Kundu,"Analgesic and anti-inflammatory potential of essential oil of Eucalyptus camaldulensis leaf: in vivo and in silico studies," Natural Product Communications, 16(4),2021. Doi:10.1177// 1934578X211007634‏.

Z. Shiguang, and S. F. Shamoun, "The effects of culture media, solid substrates, and relative humidity on growth, sporulation and conidial discharge of Valdensinia heterodoxa." Mycological research., 110.11, 1340-1346,2006. ‏

Saad El-Din Hassan,"Plant growth-promoting activities for bacterial and fungal endophytes isolated from medicinal plant of Teucrium polium L." Journal of advanced research., 8(6), 687-695, 2017.

C. Ahmet, Kordali, K. Saban, K. Hamdullah, and K. Ercan, "Antifungal properties of essential oil and crude extracts of Hypericum linarioides Bosse," Biochemical Systematics and Ecology., 33(3), 245-256, 2005.

M. Sharma, M. Sharma, and M. Sharma, M. Sharma, "Influence of culture media on mycelial growth and sporulation of some soil dermatophytes compared to their clinical isolates," 196-200, 2011.‏

T. Tsuyoshi, "Amino acid-or protein-dependent growth of Trichophyton mentagrophytes and Trichophyton rubrum," FEMS Immunology and Medical Microbiology., 29(4). 241-245, 2000.

N. Jain, "Antidermatophytic activity of some plant metabolites," Unpublished PhD. Thesis, Jaipur, India: University of Rajasthan, (2001).‏

K. Sara K., Jawad K. Al-Janabi, and Adnan H. Al-Hamadani, "In vitro, determination of optimal conditions of growth and proteolytic activity of clinical isolates of Trichophyton rubrum." Journal of contemporary medical sciences., 1.03, (2015). ‏

Aruna, G. L., and Ramalingappa, "Optimization of Environmental Parameters for Trichophyton rubrum Growth and its Antigen Production," International Journal of Health Sciences and Research., 8(1), 54 – 59, 2018.

A. Tankeshwar, and J. Hare, "Sabouraud agar and other fungal growth media." Laboratory protocols in fungal biology: current methods in fungal biology. Cham: Springer International Publishing, 69-86, 2022.‏

T. Razavyoon, S.J. Hashemi, S. Ansari, P. Mansouri, R. Daie-Ghazvini, S. Khodavaisy, Z. Raf, H.K. Sarvestani, L. Hosseinpour, p. Afshar, F. Safaie, and F. Hashemi, " Neoscytalidium dimidiatum as onychomycosis causative agent in an Iranian patient: a case report and literature review," New Microbes and New Infections., 45, 100952, 2022.

T. Emad A.a, M. Ahmed Mohamedb, L. Amany Morganc, T. Yasmin M.a, B. Radwa M.a, "Therapeutic efficacy of three systemic antifungals in onychomycosis: a randomized clinical trial," Egyptian Journal of Dermatology and Venerology., 42(1), p 45-52, Jan–Apr 2022. | DOI: 10.4103/ejdv.ejdv_4_21.

S.L. Kelly, D.C. Lamb, A.J. Corran, B.C. Baldwin, and D.E. Kelly,"Mode of action and resistance to azole antifungals associated with the formation of 14α-methylergosta-8, 24 (28)-dien-3β, 6α-diol," Biochemical and biophysical research communications., 207(3), 910-915, 1995.

B. Sara, "Essential oils: their antibacterial properties and potential applications in foods—a review," International journal of food microbiology., 94(3),223-253, 2004. ‏

Bakkali, F., Averbeck, S., Averbeck, D., and M. Idaomar, "Biological effects of essential oils–a review". Food and chemical toxicology, 46(2), 446-475, 2008.

F. D. O. Pereira, P. A. Wanderley, F. A. C. Viana, Lima, R. B. D., Sousa, F. B. D., & Lima, E. D. O. ,"Growth inhibition and morphological alterations of Trichophyton rubrum induced by essential oil from Cymbopogon winterianus Jowitt ex Bor," Brazilian Journal of Microbiology., 42, 233-242, 2011.

Sabo, Verica Aleksic, and Petar Knezevic. "Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: A review," Industrial crops and products, 132, 413-429, 2019. ‏

Elgat, W. A. A., Kordy, A. M., Bohm, M., Cerny, R., Abdel-Megeed, A., and M. Z. Salem, " Eucalyptus camaldulensis, Citrus aurantium, and Citrus sinensis Essential Oils as Antifungal Activity against Aspergillus flavus, Aspergillus niger, Aspergillus terreus, and Fusarium culmorum," Processes, 8(8), 1003, 2020.

A. Caetano, R. S., Cardoso, M. G., Resende, M. L. V., Chalfuon, S. M., Martins, M. A., Gomes, H. G., Andrade, M.E.R., Brandão, R.M., Campolina, G.A., Nelson, D. L., and J. E. de Oliveira, "Antifungal activity of poly (ε‐caprolactone) nanoparticles incorporated with Eucalyptus essential oils against Hemileia vastatrix," Letters in Applied Microbiology, 75(4), 1028-1041, 2022.

Downloads

Published

2024-09-30

Issue

Section

Biology

How to Cite

AbdHummadi, H. (2024). In vitro Investigation of the Effects of Various Media and Eucalyotus Camaldulensis Essential oil on Dermatophyte spp. Growth. Wasit Journal for Pure Sciences , 3(3), 179-185. https://doi.org/10.31185/wjps.466