The Impact of Different Mediums and The Essential Oil Eucalyotus Camaldulensis on The Growth of Aspergillus spp
DOI:
https://doi.org/10.31185/wjps.465Keywords:
Culture media, Aspergillus, Eucalyptus, Essential oilAbstract
Eucalyptus Essential oils has a beneficial properties as fungicidal, bactericidal, insecticidal and other therapeutic properties. The result also showed that the best medium for growth of isolated fungi was PDA followed by SDA, because they provide a number of nutrient that are necessary for the growth of fungi, Potatoes are a great ingredient to media like the widely used Potato Dextros Agar (PDA). These results show that the effects of the essential oils on the tested organisms increase with concentration, with the lowest radial growth recorded at 100 percent on account of A. niger (29.50 mm) and the highest at 25% on account of A. fumigatus (68.17 mm). When compared to the control, all treatments had a considerable impact on the tested fungi growth. The results also showed that A. fumigatus had a mean radial growth of (57.683 mm), whereas A. nige, with a mean radial growth of (52.583 mm), was the more sensitive fungus. A. flavus came in second with a mean radial growth of (55.042 mm). A. fumigatus had the lowest mean of inhibition percentage (43.90%) and A. niger had the greatest mean (47.83%).
References
L. F. Tan, V. L. Yap, M. Rajagopal, C. Wiart, M. Selvaraja, M.Y. Leong, and P.L. Tan, "Plant as an alternative source of antifungals against Aspergillus infections: A review". Plants., 11(22), 3009, 2022.
L. Cornelia, and M. Cuenca-Estrella,"Changes in the epidemiological landscape of invasive mould infections and disease." Journal of Antimicrobial Chemotherapy., 72.suppl_1 2017, i5-i11.
J. P. Latgé, and G. Chamilos, "Aspergillus fumigatus and Aspergillosis," Clinical microbiology reviews., 33(1), 10-1128, 2019.
G. R. Thompson, and T. F. Patterson, "Pulmonary aspergillosis". In Seminars in Respiratory and Critical Care Medicine., 29(2), 103-110, 2008.
J. W. Baddley, D. R. Andes, K. A. Marr, D. P. Kontoyiannis, B. D. Alexander, C. A. Kauffman, R. A. Oster, E. J. Anaissie, T. J. Walsh, M.G. Schuster, J. R. Wingard, T. F. Patterson, J. I. Ito., D. Williams, T. Chiller, P. G. Pappas, and Transplant Associated Infection Surveillance Network, "Factors associated with mortality in transplant patients with invasive aspergillosis," Clinical Infectious Diseases, 50(12), 1559-1567, 2010.
G. D. Brown, D. W. Denning, Gow, N. A., Levitz, S. M., Netea, M. G., and White, T. C. "Hidden killers: human fungal infections," Science translational medicine., 4(165), 165rv13-165rv13, 2012.
M. Mehani, N, Salhi, F. Dahou, S. Kasmi, I. Mehani, L. Segni, L. Morcia, C. Terzi, and M. Akram, "Antifungal, Antibacterial and Phytotoxic Activity of Essential Oil from Leaves of Eucalyptus camaldulensis," Phytothérapie., 20(1), 48-62, 2022.
A. Hawraa Faisal, Israa Ibrahim Khalil, and D. Ali Hasan Alhasan, "In vitro effects of laser radiation on the antifungal activity of Eucalyptus spp. leaves extract by chloroform," Egyptian Journal of Veterinary Sciences, 54(4), 677-688, 2023.
Elgat, W. A. A., Kordy, A. M., Böhm, M., Černý, R., Abdel-Megeed, A., amd M. Z. Salem, "Eucalyptus camaldulensis, Citrus aurantium, and Citrus sinensis Essential Oils as Antifungal Activity against Aspergillus flavus, Aspergillus niger, Aspergillus terreus, and Fusarium culmorum," Processes, 8(8), 1003, 2020.
Z. Shiguang, and Simon F. Shamoun, "The effects of culture media, solid substrates, and relative humidity on growth, sporulation and conidial discharge of Valdensinia heterodoxa," Mycological research., 110 (11), 1340-1346, 2006.
P. Kaur, Rajesh Thakur, and A. Choudhar. "An in vitro study of the antifungal activity of silver/chitosan nanoformulations against important seed borne pathogens." Int. J. Sci. Technol. Res., 1(6), 83-86, 2012.
S. K. Maheshwari, D. V. Singh, and A. K. Sahu, "Effect of several nutrient media on the growth and sporulation of Alternaria alternate," Journal of Mycopathological Research., 37, 21-23, 1999.
A. Saha, P. Mandal, S. Dasgupta, and D. Saha, "Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl," Journal of Environmental Biology. 29(3), 407-410, 2008.
Okunowo, W. O., Gbenle, G. O., Osuntoki, A. A., and Adekunle, A. A. "Media studies on Myrothecium roridum Tode: A potential biocontrol agent for water hyacinth," Journal of Yeast and Fungal Research., 1(4), 55-61, 2010.
M. E. Zain, A. A. H. H. El-Sheikh, H. G. Soliman, and A. M. Khalil, "Influence of growth medium on diagnostic characters of Aspergillus and Penicillium species," African Journal of Microbiology Research., 3(5), 280-286, 2009.
Sharma, G. P. R. R., and Pandey, R. R. "Influence of culture media on growth, colony character and sporulation of fungi isolated from decaying vegetable wastes," Journal of yeast and fungal research., 1(8), 157-164, 2010.
R.M. Ali, Sadiq, J Ahmed, Fradi, and Alaa M. Al-Aaraji. "Comparison between different cultural medium on the growth of five Aspergillus species," World Journal of Pharmaceutical Research 5 (8), 9-16, 2016.
M. Gupta, Kumari Manisha, and R. Grover, "Effect of various media types on the rate of growth of Aspergillus niger." Indian Journal of Fundamental and Applied Life Sciences 2(2),141-144, 2012.
Yuyun, F., Radix, S., Gede, S. P., Puji L., and Eryka M. "Influence of culture medium on the sporulation and viability of Aspergillus spp. and Talaromyces spp. Entomopathogenic Fungi," Journal Hama dan Penyakit Tumbuhan Tropika., 18(2), 12-22, 2018.
Dewi, P., Indrati, R., and Millati, R. "Effect of aeration on the growth and sporulation of Aspergillus niger in cassava stalks bioconversion," In Journal of Physics: Conference Series, 1918(5), 052006. IOP Publishing, 2021.
C. Viegas, M. Dias, E. Carolino, and R. Sabino, "Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence," Atmosphere.,12(1), 23, 2020.
S. A. Shafiq, A. F. Abdulkareem, and F. A. A. Shafi, "Influence of the different carbon and nitrogen sources on the production of biodiesel by oleaginous fungi Aspergillus terreus, Aspergillus fumigatus," Baghdad Science Journal., 18(2), 0225-0225, 2021.
Pujiarti, R., Nurjanto, H. H., and Sunarta, S. "Antifungal activity of Eucalyptus urophylla oil against Aspergillus niger and Fusarium oxysporum," AGRIVITA Journal of Agricultural Science., 40(1), 55-62, 2017.
Trivedi, N. A., and Hotchandani, S. C. "A study of the antimicrobial activity of oil of Eucalyptus,". Indian Journal of pharmacology., 36(2), 93-94, 2004.
G. R. Vilela, G. S. de Almeida, D'Arce, M. A. B. R., Moraes, M. H. D., Brito, J. O., da Silva, M. F., Das., G. F., and E. M. da Gloria, "Activity of essential oil and its major compound,1, 8-cineole, from Eucalyptusglobulus Labill., against the storage fungi Aspergillus flavus Link and Aspergillus parasiticus Speare,"Journal of Stored Products Research., 45(2),108-111, 2009.
G. Martin Muthee, W. Angeline Maina, and John M. Wagacha. "Antifungal activity of essential oil of Eucalyptus camaldulensis Dehnh. Against selected Fusarium spp." International journal of microbiology 2017.1, 2017. doi:10.1155/2017/8761610.
A. Valeria, L. Cavallo, J. Roana, N. Mandras, Cuffini, A. M. Tullio, V. and Banche, G. "In Vitro Antifungal Activity of Selected Essential Oils against Drug-Resistant Clinical Aspergillus spp. Strains," Molecules., 28(21), 7259, 2023.
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