The effect of Fenugreek on some biochemical parameters and histological structure of the liver in male rats induced diabetes by alloxan

Abstract

Millions of diabetics use herbal medicine for economic and cultural reasons. The
objective of the present study was to explore of the effects of fenugreek in experimental diabetic (albino) male rats induced by alloxan measuring the level of liver
enzymes Alanine transaminase, Aspartate aminotransferase and studying histological changes in the liver. The study included 40 rats divided into four groups (10 for
each group) with weights ranging from 200-250mg, the first group was as a control
group, the second group was injected with alloxan 120 /kg/b.w and nicotinamide 50
0.5 mg/kg/B.W. The third diabetic group received treatment with 50 mg/kg/b.w. of
fenugreek. The fourth diabetic group received treatment with 100 mg/kg/B.W. of
fenugreek, The animals were scarified and the heart's blood was extracted. where
the serum was isolated to study the biochemical parameters of liver enzymes, the
liver was removed and placed in 10% formalin solution for the histological study
purpose of conducting the histological study. The study's findings demonstrated an
increase in the effectiveness of liver enzymes as a result of the injection of alloxan,
but after treatment with fenugreek, the level of these enzymes decreased in the third
and fourth groups and also demonstrated that induction of diabetes caused changes
in the rats livers, as it showed congestion of blood vessels in the hepatic central
vein, necrosis of hepatocytes, irregular sinusoids, and thickening of the nuclei in
diabetic group.