Isolation and Identification of Staphylococcus Aureus from Al-Kut Hospital in Kut City

Abstract

Samples were collected from the hospital in 2019-2020, where the collection and diagnosis of samples about period from 2019/11 to the last diagnosis in the laboratory 2020/2/1, samples were harvested from patient rooms, waiting rooms and door handles. The samples were isolated with 100 samples and placed in special tubes to bacteriology laboratory. Isolation and diagnosis of bacteria include grow in different media like nutrient and Mannitol Salt agar  and in selective Medea like Baird Parker Agar with staining with gram stain to show bacteria shape and characteristic. The antibiotic's sensitivity to these bacteria was detected by observing and measuring the area of inhibition of bacterial growth with the presence of different types of antibiotics. The purpose of this research to know the amount of pathological bacteria present in the hospital and classify them for the purpose of avoiding the danger to human life, to control them and diagnose them in easy and quick ways, and to know their sensitivity against different types of antibiotics.