Assessment of Tissue inhibitor of metalloproteinase-1 and many biochemical parameters in patients with chronic hepatitis B in Tikrit city

Abstract

Abstract— The main objective of this cross-sectional investigation was to assess biomarkers associated with liver fibrosis, namely Tissue inhibitor of metalloproteinase 1 TIMP-1, Glutamate-pyruvic transaminase GPT, glutamic-oxaloacetic transaminase GOT, and Alkaline phosphatase ALP. which conduct in Tikrit University, College of Science /Biology Department the study samples collected from  patients in Dialysis Unit in Tikrit Teaching Hospital, ninty subjects where sub-divided to 60 patients of age (20– 68) years, the Ages of Patients Chronic hepatitis B Ranged From (20-67) years( 19 males and 11 females ) And The Ages Of Patients with chronic hepatitis B  suffered of chronic kidney diseases  (24-68) Years ( 18males and 12 females) and 30 as control group aged (20-67) years, in this investigation Enzyme linked immunosorbent assay (ELISA) kites were employed to assess serum concentrations of (TIMP-1), Whereas used Monarch-240 to detect the activity of enzymes such as GPT, GOT and ALP.

The findings of the present investigation demonstrated in chronic hepatitis patients there were significant increased (P≤0.05) in the levels of TIMP-1, and activity of GPT and ALP whereas no significant rise (P≥0.05) for activity of GOT when compared with control.   In patients with chronic hepatitis B (CHB) suffered of chronic kidney diseases(CKD) there were significant raised (P<0.05) in the levelsTIMP-1, ALT and ALP while no significant rised (P≥0.05) in levels of AST when compared with control.

Current data suggest that TIMP-1 can be a promising biomarker of active fibrogenesis, after HBV chronic infection.