Correlation between interleukin-6 and endotoxin an in vitro study
DOI:
https://doi.org/10.31185/wjps.183Keywords:
interleukin-6 (IL-6), bacterial endotoxin, in vitro study, immune response, inflammation, peripheral blood mononuclear cells, cell culture, ElisaAbstract
Endotoxin, a component of the outer membrane of Gram-negative bacteria, has been extensively studied as a stimulator of the innate immune response. However, the temporal aspects and exposure-response relationship of endotoxin and resulting cytokine induction. This in vitro study aimed to investigate the factors influencing the interleukin-6 (IL-6) response to bacterial endotoxin stimulation. IL-6 is a pro-inflammatory cytokine that plays a crucial role in the immune response and inflammation. In this study, we utilized an in vitro model to examine the factors influencing IL-6 production in response to bacterial endotoxin this experimental study was performed to find out the effect of healthy person on peripheral blood mononuclear cells(PBMCs) namely T-cells and natural killer cells(NK cells) function through challenging their ability for cytokines production in a comparative study by using of standardized inoculum of Bacterial endotoxins.
References
Nakamori, Y., E.J. Park, and M.J.F.i.i. Shimaoka, Immune deregulation in sepsis and septic shock: reversing immune paralysis by targeting PD-1/PD-L1 pathway. 2021. 11: p. 624279.
Jean Beltran, P.M., et al., Proteomics and integrative omic approaches
for understanding host–pathogen interactions and infectious diseases. 2017. 13(3): p. 922.
Aleruchi, C., et al., African Journal of Biological Sciences. 2022 .3
Kleiveland, C.R.J.T.I.o.F.B.o.H.i.v. and e.v. models, Peripheral blood mononuclear cells. 2015: p. 161-167.
Li, L., et al., Effects of immune cells and cytokines on inflammation and immunosuppression in the tumor microenvironment. 2020. 88: p. 106939.
Marshall, J.S., et al., An introduction to immunology and immunopathology. 2018. 14(2): p. 1-10.
Qian, L., et al., Safinamide prevents lipopolysaccharide (LPS)-induced inflammation in macrophages by suppressing TLR4/NF-κB signaling. 2021. 96: p. 107712.
Gorman, A., A.P.J.E.J.o.P. Golovanov, and Biopharmaceutics, Lipopolysaccharide Structure and the Phenomenon of Low Endotoxin Recovery. 2022.
Mohammad, S. and C.J.F.i.i. Thiemermann, Role of metabolic endotoxemia in systemic inflammation and potential interventions. 2021. 11: p. 594150.
David, C.A., et al., In vitro determination of the immunogenic impact of nanomaterials on primary peripheral blood mononuclear cells. 2020. 21(16): p. 5610.
Patrone, D., et al., Optimization of Peripheral Blood Mononuclear Cell Extraction from Small Volume of Blood Samples: Potential Implications for Children-Related Diseases. 2022. 5(2): p. 20.
Saferding, V., et al., A6. 42 The role of micro-RNA-146a in inflammatory arthritis. 2015, BMJ Publishing Group Ltd.
Bercusson, A., Defining the impact of Ibrutinib therapy and CFTR dysfunction on macrophage function in the innate immune response to Aspergillus fumigatus. 2019.
Wang, Z., et al., A novel oriented immunosensor based on AuNPs-thionine-CMWCNTs and staphylococcal protein A for interleukin-6 analysis in complicated biological samples. 2020. 1140: p. 145-152.
Tanaka, T., M. Narazaki, and T.J.C.S.H.p.i.b. Kishimoto, IL-6 in inflammation, immunity, and disease. 2014. 6(10): p. a016295.
Thorsted, A., et al., A non-linear mixed effect model for innate immune response: In vivo kinetics of endotoxin and its induction of the cytokines tumor necrosis factor alpha and interleukin-6. 2019. 14(2): p. e0211981.
Veiz, E., et al., Increased Concentrations of Circulating Interleukins following Non-Invasive Vagus Nerve Stimulation: Results from a Randomized, Sham-Controlled, Crossover Study in Healthy Subjects. 2022. 29(4): p. 450-459.
Liu, Y., et al., 6-Gingerol attenuates microglia-mediated neuroinflammation and ischemic brain injuries through Akt-mTOR-STAT3 signaling pathway. 2020. 883: p. 173294.
Hua, Y., et al., Exposure to hydroxyapatite nanoparticles enhances Toll-like receptor 4 signal transduction and overcomes endotoxin tolerance in vitro and in vivo. 2021. 135: p. 650-662.
Saia, R.S., A.B. Ribeiro, and H.J.S. Giusti, Cholecystokinin modulates the mucosal inflammatory response and prevents the lipopolysaccharide-induced intestinal epithelial barrier dysfunction. 2020. 53(2): p. 242-251.
Üçeyler, N., et al., Tumor necrosis factor-α links heat and inflammation with Fabry pain. 2019. 127(3): p. 200-206
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Sawsan Mohsen
This work is licensed under a Creative Commons Attribution 4.0 International License.
