The effect of phytoestrogen (lignan) on the levels of some hormo-nal parameters in white female rats induced osteoporosis

Abstract

The current study aimed to evaluate the effectiveness of the phytoestrogen (lignan) in some hormonal parameters  of white female rats induced osteoporosis with Prednisolone. The present study included two main experiments, In the first experiment 50 adult white female rats were divided into two groups, The first group G1  was considered a control group and the second group, G2 was induced into osteoporosis by Prednisolone at a dose of 40 mg/kg of body weight For two months. The second main experiment divided the remaining animals (from the first group of the first experiment into three subgroups) and the remaining animals (from the second group of the first experiment into two subgroups): negative control group C was dosed with distilled water, group T1 was considered a positive control that was continued with Prednisolone at a dose of 40 mg/kg of body weight, group T2,  was induced osteoporosis and administered the phytoestrogens(lignan) at a dose of 20 mg/kg,  the healthy T3 group was administered the phytoestrogens(lignan) at a dose of 20 mg/kg, and the T4 group was administered the phytoestrogens(lignan) at a dose of 20 mg/kg. And Prednisolone at a dose of 40 mg/kg simultaneously for 30 days. After the end of the second experiment, the weights of the animals were recorded and then anesthetized with chloroform withdrawal blood samples from the heart directly for hormonal tests that represent estrogen, FSH, LH. The results showed a significant decrease (P<0.05) in each of the average estrogen, FSH, LH  level in the T1 group (positive control) when comparing to the negative group. while the cause of the  phytoestrogen(lignan) improved The hormonal parameters   and showed a significant increase (P<0.05) in estrogen, FSH, LH level  in T2,T3,T4groups when comparing to T1 (positive control). We conclude from the current study the effective and positive role shown by phytoestrogens(lignan) in improving and enhancing the  hormonal  parameters and reducing the negative effects of induced osteoporosis in white female rats.