Detection of some virulence genes of Staphylococcus aureus that caused Infective Endocarditis

Abstract

Infective Endocarditis (IE), first documented over 350 years ago, is condition characterized by infection of the endocardial membrane of the heart. The clinical presentations of irreversible endocarditis (IE) can affect all organ systems, with the cardiac manifestations including valve vegetation, abscess, periannular extension of infection, and myopericarditis. 230 Blood sample were collected from Infective Endocarditis patients, blood culture used for bacterial growth and identification of bacterial species were completed by microscopic examination, culture characteristics, and biochemical tests, and the use of the Vitek 2 diagnostic system for final identification of the isolated bacteria. Antibiotic sensitivity test for S. aureus were performed by disk diffusion method , mecA and  sarA genes were detected by PCR. 51 positive culture for bacterial growth and 18 were S. aureus bacteria, most of Staph aureus were resistant to ampicillin (100%), amoxiclav (83.3%)and pencillin(94.4%) while they were highly sensitive to Azithromycin, Cefotaxime, Ceftriaxone, Gentamicin, Imipenem, Levofloxacin, Amikacin, and Trimethoprim, which reached 72.22%, 72.22%, 83.33%, 88.89%, 83.33%, and 88.8% 9, 83.33%, 83.33%, 72.22%, and 88.89%, respectively. mecA gene were found in (42,8%)of S. aureus isolates while sarA gene were found in (64.2%). S. aureus have many virulence factors genes that contributed in the infective Endocarditis infection and they were resistant to many antibiotics due to these genes.