Green Synthesis of Silver Nitrate Nanoparticles from E. coli and Evaluation of Their Inhibitory Effectiveness against E. coli, S. aureus, and B. subtilis
DOI:
https://doi.org/10.31185/wjps.389Keywords:
Green synthesis, Silver nanoparticles, X-ray diffraction, scanning electron microscopyAbstract
this study investigated the environmentally friendly production of silver nitrate nanoparticles (Agno3 NPs) utilizing E.coli and assesses their ability to kill harmful bacteria such as E.coli , Staphylococcus aureus , and bacillus subtilis employing biological processes for nanoparticles synthesis offers both ecological advantages and improved compatibility with biological system the biogenic synthesis entailed cultivating E.coli in tryptic soya broth, and subsequently treating it with silver nitrate solution to generate AgNPs the produced nanoparticles underwent characterization using scanning electrone microscope (SEM), X-ray diffraction (XRD) and energy dispersive X ray (EDX) examination the antibacterial efficacy was evaluated by quantifying the zone of inhibition diameter against the microorganisms being tested, indicating that higher quantities of AgNPS where associated with greater antibacterial potency . this study emphasizes the possibility of utilizing biologically produced nanoparticles in medical applications, namely as a substitute for conventional antibacterial treatment which frequently encounter challenges related to resistance
References
R. Ahmad, N. Aramesh, S. Hasnain, A. K. Nayak, and R. Varma, "Greener fabrication of metal
nanoparticles using plant materials: A review," Chemical Physics Impact, vol. 2023, Art. no. 100255,
, doi: 10.1016/j.chphi.2023.100255.
R. Bishwas, S. Jahan, and M. A. Alam, "An Investigation on Synthesis of Silver Nanoparticles,"
Asian Journal of Research in Biochemistry, vol. 12, pp. 1-10, 2023, doi:
9734/ajrb/2023/v12i3234.
W. Zhang, G. Ye, D. Liao, X. Chen, C. Lu, A. Nezamzadeh-Ejhieh, M. S. Khan, J. Liu, Y. Pan,
and Z. R. Dai, "Recent Advances of Silver-Based Coordination Polymers on Antibacterial
Applications," Molecules, vol. 27, no. 27, Art. no. 2717166, 2022, doi: 10.3390/molecules27217166.
A. J. Jasem and M. A. Mahmood, "Preparation and Characterization of Amoxicillin-loaded
Chitosan Nanoparticles to Enhance Antibacterial Activity against Dental Decay Pathogens," Journal
of Emergency Medicine, Trauma & Acute Care, vol. 3, Art. no. 10, 2023,
https://doi.org/10.5339/jemtac.2023.midc.10.
R. Devadharshini, G. Karpagam, K. Pavithra, S. Kowsalya, P. P. Mohana, and R. Mohanram,
"Green Synthesis of Silver Nanoparticles," Microbiology Research Journal International, vol. 33, pp.
-9, 2023, doi: 10.9734/mrji/2023/v33i51380.
V. Maharani, A. Sundaramanickam, and T. Balasubramanian, "In vitro anticancer activity of
silver nanoparticle synthesized by Escherichia coli VM1 isolated from marine sediments of Ennore
southeast coast of India," Enzyme Microb Technol., vol. 95, pp. 146-154, Dec. 2016, doi:
1016/j.enzmictec.2016.09.008.
Dr. M. Ramaiah, S. Ignacimuthu, a. J. a. Ranjitsingh, R. Krishnamoorthy, and M. Paulraj, "Culture
Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles,"
Advances in Materials Science and Engineering, vol. 2023, pp. 1-6, 2023, doi:
1155/2023/1680311.
O. Daramola, N. Torimiro, and S. Alayande, "Affinity capture of Escherichia coli pathotypes
using poly-L-lysine functionalized silver nanoparticles," Advances in Natural Sciences: Nanoscience
and Nanotechnology, vol. 13, Art. no. 025012, 2022, doi: 10.1088/2043-6262/ac7712.
B. Essghaier, H. Hannachi, R. Nouir, F. Mottola, and L. Rocco, "Green Synthesis and
Characterization of Novel Silver Nanoparticles Using Achillea maritima subsp. maritima Aqueous
Extract: Antioxidant and Antidiabetic Potential and Effect on Virulence Mechanisms of Bacterial
and Fungal Pathogens," Nanomaterials (Basel), vol. 13, no. 13, Art. no. 1964, Jun. 28, 2023, doi:
3390/nano13131964
N. González-Ballesteros, M. Fernandes, R. Machado, P. Sampaio, A. C. Gomes, A. Cavazza, F.
Bigi, and M. C. Rodríguez-Argüelles, "Valorisation of the Invasive Macroalgae Undaria pinnatifida
(Harvey) Suringar for the Green Synthesis of Gold and Silver Nanoparticles with Antimicrobial and
Antioxidant Potential," Marine Drugs, vol. 2023, doi: 10.3390/md21070397, 2023.
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