Effect of Monosodium Glutamate on Serum Neurotransmitters and the Protective Role of Vitamin B12 in Adult Female Rats

Authors

  • Zainab Wisam Ministry of Education

DOI:

https://doi.org/10.31185/wjps.318

Abstract

Glutamate is one of the most exciting neurotransmitters in the brain. The current study investigates the effect of Monosodium glutamate (MSG) on Serum level of that include, glutamate, acetylcholinesterase, epinephrine and serotonin protective effect of vitamin B12. Rats were divided into five equal groups n=10, each group was further divided into two groups (A and B, n=5) and treated for 30 and 60 days, respectively. The 1st group received distilled water orally by gavage, the 2nd and 3rd group received MSG in a dose of 20, and 40 mg/kg body weight orally by gavage, respectively, the 4th and 5th groups received MSG and vitamin B12 in a dose of 20 or 40 mg MSG/kg in addition to 0.3m vitamin B12/kg, respectively. Monosodium glutamate causes a significant reduction in serum level of neurotransmitters. These changes were reversed by treatment with vitamin B12. There was improvement in the levels of neurotransmitters after treatment with vitamin B12

References

J. F. B. Morrison, S. Shehab, R. Sheen, S. Dhanasekaran, M. Shaffiullah, and E. Mensah‐Brown, “Sensory and autonomic nerve changes in the monosodium glutamate‐treated rat: a model of type II diabetes,” Exp. Physiol., vol. 93, no. 2, pp. 213–222, 2008.

M. R. Al-Attabi, N. A. K. Alzubaidi, and N. H. Al-Moussawi, “Effect of Dietary Monosodium Glutamate on Some Dipose-Derived Hormones in Adult Male Rats,” J. Adv. Biol., vol. 7, no. 2, pp. 1268–1274, 2015.

V. Sharma and R. A. Deshmukk, “A fifth taste or bio bomb,” Euro J Pharma Med Res, vol. 2, no. 2, pp. 381–400, 2015.

R. S. Geha et al., “Review of alleged reaction to monosodium glutamate and outcome of a multicenter double-blind placebo-controlled study,” J. Nutr., vol. 130, no. 4, pp. 1058S-1062S, 2000.

J. F. B. Morrison, S. Shehab, R. Sheen, S. Dhanasekaran, M. Shaffiullah, and E. Mensah‐Brown, “Sensory and autonomic nerve changes in the monosodium glutamate‐treated rat: a model of type II diabetes,” Exp. Physiol., vol. 93, no. 2, pp. 213–222, 2008.

A. Ç. Kardeşler and E. BAŞKALE, “Investigation of the behavioral and neurochemical effects of monosodium glutamate on neonatal rats,” Turkish J. Med. Sci., vol. 47, no. 3, pp. 1002–1011, 2017.

L. J. Filer and L. D. Stegink, “Report of the proceedings of the glutamate workshop,” Crit. Rev. Food Sci. Nutr, vol. 34, no. 2, pp. 159–174, 1994.

S. S. Schiffman and J. M. Gill, “Psychophysical and neurophysiological taste responses to glutamate and purinergic compounds,” Umami, a basic Tast. New York Marcel Dekker, pp. 271–288, 1987.

T. Yamamoto, R. Matsuo, Y. Fujimoto, I. Fukunaga, A. Miyasaka, and T. Imoto, “Electrophysiological and behavioral studies on the taste of umami substances in the rat,” Physiol. Behav., vol. 49, no. 5, pp. 919–925, 1991.

E. O. Farombi and O. O. Onyema, “Monosodium glutamate-induced oxidative damage and genotoxicity in the rat: modulatory role of vitamin C, vitamin E and quercetin,” Hum. Exp. Toxicol., vol. 25, no. 5, pp. 251–259, 2006.

Adrienne S. The toxicity-Safety of MSG. Acct Res. 1999;6(4):259–310.

S. G. Rosa, P. M. Chagas, A. P. Pesarico, and C. W. Nogueira, “Monosodium glutamate induced nociception and oxidative stress dependent on time of administration, age of rats and susceptibility of spinal cord and brain regions,” Toxicol. Appl. Pharmacol., vol. 351, pp. 64–73, 2018.

J. Löliger, “Function and importance of glutamate for savory foods,” J. Nutr., vol. 130, no. 4, pp. 915S-920S, 2000.

M. Mohan, S. Gangurde, and V. Kadam, “Protective effect of Solanum torvum on monosodium glutamate-induced neurotoxicity in mice,” 2017.

B. D. Shivasharan, P. Nagakannan, B. S. Thippeswamy, and V. P. Veerapur, “Protective effect of Calendula officinalis L. flowers against monosodium glutamate induced oxidative stress and excitotoxic brain damage in rats,” Indian J. Clin. Biochem., vol. 28, no. 3, pp. 292–298, 2013.

R. Green et al., “Vitamin B 12 deficiency,” Nat. Rev. Dis. Prim., vol. 3, no. 1, pp. 1–20, 2017.

O. J. Onaolapo, A. Y. Onaolapo, M. A. Akanmu, and O. Gbola, “Evidence of alterations in brain structure and antioxidant status following ‘low-dose’monosodium glutamate ingestion,” Pathophysiology, vol. 23, no. 3, pp. 147–156, 2016.

C. O. Edoga, J. E. Ejo, C. A. Anukwuorji, C. E. Ani, and M. I. Izundu, “Antioxidant Effects of Mixed Doses of Vitamins B12 and E on Male Wistar Albino Rats Infected with Trypanosoma Brucei Brucei,” Immunol. Infect. Dis., vol. 8, no. 2, pp. 7–13, 2020.

M. F. Taleb and A. H. Ulaiwi, “DETECTION OF INSULIN SECRETION AND LIPID PROFILE FOR CHROMIUM-SUPPLEMENTS AGAINST H5 INFLUENZA VACCINATION IN LAYERS,” Iraqi J. Agric. Sci., vol. 54, no. 5, pp. 1292–1297, 2023.

R. Z. Hamza, F. A. Al-Salmi, and N. S. El-Shenawy, “Evaluation of the effects of the green nanoparticles zinc oxide on monosodium glutamate-induced toxicity in the brain of rats,” PeerJ, vol. 7, p. e7460, 2019.

H. N. Saada, U. Z. Said, S. M. Shedid, E. M. E. Mahdy, and H. E. Elmezayen, “Influence of Monosodium Glutamate on Radiation-Induced Biochemical Alterations in Male Albino Rats,” J. Nucl. Technol. Appl. Sci., vol. 2, 2014.

O. O. Onyema, E. O. Farombi, G. O. Emerole, A. I. Ukoha, and G. O. Onyeze, “Effect of vitamin E on monosodium glutamate induced hepatotoxicity and oxidative stress in rats,” 2006.

J. M. Gebicki, “Oxidative stress, free radicals and protein peroxides,” Arch. Biochem. Biophys., vol. 595, pp. 33–39, 2016.

J. J. Ramos-Rodriguez, S. Molina-Gil, R. Rey-Brea, E. Berrocoso, and M. Garcia-Alloza, “Specific serotonergic denervation affects tau pathology and cognition without altering senile plaques deposition in APP/PS1 mice,” PLoS One, vol. 8, no. 11, p. e79947, 2013.

A. M. Waggas, “Neuroprotective evaluation of extract of ginger (Zingiber officinale) root in monosodium glutamate-induced toxicity in different brain areas male albino rats.,” Pak. J. Biol. Sci., vol. 12, no. 3, pp. 201–212, 2009.

O. J. Onaolapo, O. S. Aremu, and A. Y. Onaolapo, “Monosodium glutamate-associated alterations in open field, anxiety-related and conditioned place preference behaviours in mice,” Naunyn. Schmiedebergs. Arch. Pharmacol., vol. 390, no. 7, pp. 677–689, 2017.

U. K. Hussein et al., “Ginger and propolis exert neuroprotective effects against monosodium glutamate-induced neurotoxicity in rats,” Molecules, vol. 22, no. 11, p. 1928, 2017.

I. S. Olateju, O. J. Olarotimi, O. A. Adu, and F. A. Gbore, “Effects of dietary monosodium glutamate on the acetylcholinesterase, specific acetylcholinesterase and total protein concentrations in the brain regions of domestic fowl (Gallus domesticus) layers,” Niger. J. Anim. Sci., vol. 21, no. 3, pp. 89–94, 2019.

S. Umukoro, G. O. Oluwole, H. E. Olamijowon, A. I. Omogbiya, and A. T. Eduviere, “Effect of monosodium glutamate on behavioral phenotypes, biomarkers of oxidative stress in brain tissues and liver enzymes in mice,” World J. Neurosci., vol. 5, no. 05, p. 339, 2015.

E. R. Youness, J. S. Hussein, A. M. M. Ibrahim, and F. E. Agha, “Flaxseed oil attenuates monosodium glutamate-induced brain injury via improvement of fatty acids contents,” Biomed. Pharmacol. J., vol. 12, no. 2, pp. 527–532, 2019.

S. M. Hazzaa, S. A. M. Abdelaziz, M. A. Abd Eldaim, M. M. Abdel-Daim, and G. E. Elgarawany, “Neuroprotective potential of allium sativum against monosodium glutamate-induced excitotoxicity: impact on short-term memory, gliosis, and oxidative stress,” Nutrients, vol. 12, no. 4, p. 1028, 2020.

S. Padmanabhan et al., “Folate/Vitamin B12 supplementation combats oxidative stress-associated carcinogenesis in a rat model of colon cancer,” Nutr. Cancer, vol. 71, no. 1, pp. 100–110, 2019.

K. Suzuki et al., “Electrospun nanofiber sheets incorporating methylcobalamin promote nerve regeneration and functional recovery in a rat sciatic nerve crush injury model,” Acta Biomater., vol. 53, pp. 250–259, 2017.

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Published

2024-03-30

Issue

Vol. 3 No. 1 (2024)

Section

Biology

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Copyright (c) 2024 Zainab Wisam

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How to Cite

Wisam, Z. (2024). Effect of Monosodium Glutamate on Serum Neurotransmitters and the Protective Role of Vitamin B12 in Adult Female Rats. Wasit Journal for Pure Sciences , 3(1), 201-208. https://doi.org/10.31185/wjps.318